1. （From Osaka Station) Take the JR Osaka Loop Line (inner track) to Bentencho. Tip: The inner track runs counter clockwise, and the outer track runs clockwise, but even if you make a mistake, it`s a circle so you`ll eventually get there. 2.(From Bentencho Station) transfer to the Subway Chuo (Cetral) Line and go to Osakako Station. From Osakako Station, it`s a 5 minute walk north west to the aquarium.
About 30 minutes from Osaka Station (including the walk)